Investigation of The Antibiotic Biosynthetic
vor 5 Monaten
**Masterarbeit**
**Investigation of the antibiotic biosynthetic potential of **_Kitasatospora fiedleri _**DSM 114396**
**Background**
Actinomycetes are the origin of almost two thirds of all antibiotics in clinical use today, and still the most promising source for novel natural compounds with antimicrobial activity. While the emergence of multidrug-resistant pathogens is constantly increasing, there has been a steady decline in the discovery of new and effective antibiotics over the past decades. Thus, there is an urgent need to find new anti-infectives to combat drug resistance. _Kitasatospora fiedleri _DSM 114396 (a rare genus and novel species of the family _Streptomycetaceae_) is a strain from the DSMZ strain collection that produces one or more antimicrobials of unknown structure and biochemistry.
In actinomycetes, the genes encoding the biosynthesis of natural products are usually located in a continuous region of the genome we refer to as biosynthetic gene cluster (BGC). The _Kitasatospora _genome harbours >25 BGCs most of them cryptic (without known metabolic product). Genetic manipulation of the strains has been successfully achieved in our laboratory.
**Thesis Topic**
The ultimate goal of the project is to identify the BGCs driving the production of the different antimicrobial compounds. This will be achieved by step-wise inactivation of selected BGCs by genetic manipulation of the producer strain, using a variation of CRISPR-Cas9 technology known as CRISPR-base editing systems; establishing this technique in our lab, and testing with one BGC, will be the objective of the Master Thesis.
**_ Experiments_**_:_
- Selection of target genes from prioritised BGCs identified by antiSMASH analysis;
- Design of possible genome edits to make and required CRISPR spacers
- Construction of CRISPR delivery vectors and conjugation to _Kitasatospora_;
- Assessment of antimicrobial activity produced by mutants.
**_Methodology_**_:_
Bioinformatics (database search, analysis of DNA sequences, planning of DNA cloning). Molecular biology (DNA extraction and purification, PCR amplification, DNA cloning, _E.coli _transformation, restriction analysis). Microbiology (culture and preservation of microorganisms). Antimicrobial activity bioassays. Genetic manipulation of microorganisms (mobilisation of DNA into _Kitasatospora_, homologous recombination-based gene targeting, selection of mutants)
**Who we are**
The group _Applied Microbiology_ _(head: Prof. Yvonne Mast), located at the Leibniz-Institut DSMZ in Braunschweig-Stöckheim, investigates actinomycetes as antibiotic producers. We employ Genome Mining and Genetic Engineering techniques to fully exploit the biosynthetic potential of actinomycetes.
**Start: immediately**
Download this announcement as PDF
-
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob Title: Electrical Teacher: Lawrenceville Correctional Center #ED008The Commonwealth of Virginia Department of Corrections is seeking a highly qualified Electrical Teacher to join our team at the Lawrenceville Correctional Center. As an Electrical Teacher, you will be responsible for providing effective Career and Technical instruction in the assigned...
-
Polyphasic Taxonomic Description and Evaluation of
vor 5 Monaten
Braunschweig, Deutschland Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH Vollzeit**Masterarbeit** **P**olyphasic taxonomic description and evaluation of biotechnological potential of two novel **_Streptomyces_** species**: **Background** Modern prokaryotic systematics based on a polyphasic approach has allowed to resolve the taxonomic status of several heterogenous complex actinobacterial taxa. Strains of the phylum _Actinomycetota_ are...
-
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryThe Inmate Grievance Coordinator is responsible for monitoring the grievance procedure in its entirety, ensuring compliance with VADOC policy. This role conducts investigations of inmate grievances, consults with staff and inmates to resolve problems/issues, and prepares responses for correspondence as required by the Operations Manager.Key...
-
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryThe Inmate Grievance Coordinator is responsible for monitoring the grievance procedure in its entirety, ensuring compliance with VADOC policy. This role conducts investigations of inmate grievances, consults with staff and inmates to resolve problems/issues, and prepares responses for correspondence as required by the Operations Manager.Key...
-
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryThe Commonwealth of Virginia is seeking a highly skilled Inmate Grievance Coordinator to join our team. As a key member of our operations, you will be responsible for monitoring the grievance procedure, conducting investigations, and providing guidance to inmates.Key ResponsibilitiesMonitor the grievance procedure to ensure compliance with VADOC...
-
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryThe Commonwealth of Virginia is seeking a highly skilled Inmate Grievance Coordinator to join our team. As a key member of our operations, you will be responsible for monitoring the grievance procedure, conducting investigations, and providing guidance to inmates.Key ResponsibilitiesMonitor the grievance procedure to ensure compliance with VADOC...
-
Xylitol Metabolism in Phaeobacter Inhibens
vor 2 Monaten
Braunschweig, Deutschland Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH Vollzeit**Masterarbeit** **Xylitol metabolism in **_Phaeobacter inhibens_** **Background** Members of the Roseobacter group are some of the most prevalent prokaryotes in marine ecosystems. Known for their remarkable metabolic versatility, these bacteria play a significant role in global marine biogeochemical cycles. Although well-established model organisms like...
-
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryThe Commonwealth of Virginia is seeking a highly skilled Inmate Grievance Coordinator to join our team at the Lawrenceville Correctional Center. As a key member of our operations team, you will be responsible for ensuring compliance with VADOC policy and procedures related to inmate grievances.Key ResponsibilitiesMonitor and investigate inmate...
-
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryThe Commonwealth of Virginia is seeking a highly skilled Inmate Grievance Coordinator to join our team at the Lawrenceville Correctional Center. As a key member of our operations team, you will be responsible for ensuring compliance with VADOC policy and procedures related to inmate grievances.Key ResponsibilitiesMonitor and investigate inmate...
-
HVAC Teacher for Virginia Department of Corrections
vor 3 Wochen
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob Summary:We are seeking a highly qualified HVAC educator to join our team at the Virginia Department of Corrections. As an HVAC instructor, you will be responsible for providing effective career and technical instruction in the assigned trade area, assisting students in completing required competencies, and demonstrating effective classroom or lab...
-
Lawrenceville Correctional Center Position
vor 3 Wochen
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryWe are seeking a highly skilled Inmate Grievance Coordinator to join our team at the Lawrenceville Correctional Center. The successful candidate will be responsible for monitoring the grievance procedure, conducting investigations, and providing guidance to inmates.Key ResponsibilitiesMonitor the grievance procedure to ensure compliance with VADOC...
-
Lawrenceville Correctional Center Position
vor 3 Wochen
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryWe are seeking a highly skilled Inmate Grievance Coordinator to join our team at the Lawrenceville Correctional Center. The successful candidate will be responsible for monitoring the grievance procedure, conducting investigations, and providing guidance to inmates.Key ResponsibilitiesMonitor the grievance procedure to ensure compliance with VADOC...
-
Grievance Resolution Specialist
vor 3 Wochen
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryWe are seeking a highly skilled Inmate Grievance Coordinator to join our team at the Lawrenceville Correctional Center. The successful candidate will be responsible for monitoring the grievance procedure, conducting investigations, and providing guidance to inmates.Key ResponsibilitiesMonitor the grievance procedure to ensure compliance with VADOC...
-
Grievance Resolution Specialist
vor 3 Wochen
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryWe are seeking a highly skilled Inmate Grievance Coordinator to join our team at the Lawrenceville Correctional Center. The successful candidate will be responsible for monitoring the grievance procedure, conducting investigations, and providing guidance to inmates.Key ResponsibilitiesMonitor the grievance procedure to ensure compliance with VADOC...
-
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryWe are seeking a highly skilled Inmate Grievance Coordinator to join our team at the Lawrenceville Correctional Center. The successful candidate will be responsible for monitoring the grievance procedure, conducting investigations, and providing guidance and consultation to inmates.Key ResponsibilitiesMonitor the grievance procedure to ensure...
-
Grievance Resolution Specialist
vor 3 Wochen
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryWe are seeking a highly skilled and detail-oriented Inmate Grievance Coordinator to join our team at the Lawrenceville Correctional Center. The successful candidate will be responsible for monitoring the grievance procedure, conducting investigations, and providing guidance and consultation to inmates.Key ResponsibilitiesMonitor the grievance...
-
Inmate Grievance Coordinator
vor 3 Wochen
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryWe are seeking a highly skilled Inmate Grievance Coordinator to join our team at the Lawrenceville Correctional Center. The successful candidate will be responsible for monitoring the grievance procedure, conducting investigations, and providing guidance and consultation to inmates.Key ResponsibilitiesMonitor the grievance procedure to ensure...
-
Inmate Grievance Coordinator
vor 3 Wochen
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryWe are seeking a highly skilled Inmate Grievance Coordinator to join our team at the Lawrenceville Correctional Center. The successful candidate will be responsible for monitoring the grievance procedure, conducting investigations, and providing guidance and consultation to inmates.Key ResponsibilitiesMonitor the grievance procedure to ensure...
-
Correctional Facility Grievance Manager
vor 3 Wochen
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryWe are seeking a highly skilled Inmate Grievance Coordinator to join our team at the Lawrenceville Correctional Center. The successful candidate will be responsible for monitoring the grievance procedure, conducting investigations, and providing guidance and consultation to inmates.Key ResponsibilitiesMonitor the grievance procedure to ensure...
-
Institutional Laundry Supervisor
vor 3 Wochen
Braunschweig, Niedersachsen, Deutschland Commonwealth of Virginia VollzeitJob SummaryWe are seeking a highly skilled and experienced Laundry Operations Manager to oversee the daily operations of our institutional laundry facility. The successful candidate will be responsible for managing the laundry process, supervising inmate workers, and ensuring the highest level of quality and efficiency in our operations.Key...
