Polyphasic Taxonomic Description and Evaluation of
vor 6 Monaten
**Masterarbeit**
**P**olyphasic taxonomic description and evaluation of biotechnological potential of two novel **_Streptomyces_** species**:
**Background**
Modern prokaryotic systematics based on a polyphasic approach has allowed to resolve the taxonomic status of several heterogenous complex actinobacterial taxa. Strains of the phylum _Actinomycetota_ are of agricultural, biotechnological, ecological, and medicinal interest. Two-thirds of all clinically relevant antibiotics, as well as many anticancer, antifungal and immunosuppressive agents are derived from actinomycetes. The choice of the biological material can be crucial for the success rate of finding new natural products and that a new source and/or biology leads to new chemical entities. In view of the continued emergence of drug-resistant bacteria associated with life-threatening infections, there is an urgent need for new antibiotics as products from taxonomically unique actinobacterial strains. In this respect, it is important to enhance the diversity of _Actinobacteria_ taxa by describing novel species of Streptomyces, as strains of this genus have been served as producers not only hundreds of antibiotics, but also enzymes, nanoparticles, or other industrially or pharmaceutically valuable biomolecules.
**Thesis Topic**
The research project consists of two work packages (WP): (WP1) polyphasic taxonomic studies of _Streptomyces _strains isolated from German soil samples and (WP2) evaluation of their biotechnological potential based on _in vitro_ and _in silico_ analyses.
**Experiments**:
- Phylogeny based on 16S rRNA gene and genome sequences (database search, analysis of DNA sequences)
- Growth and culture properties of the strains (media, pH, temperature, salinity tests)
- Biochemical and enzymatic tests (API zym, catalase, oxidase etc)
- Genome mining based on Antismash
- Antimicrobial activity bioassays
**Methodology**:
- Culture-dependent approach (selective isolation of _Actinobacteria_ strains from environmental samples, colour grouping)
- Modern Taxonomy (chemotaxonomic analysis, phylogeny based on single gene and genome sequences)
- Bioinformatics (database search, analysis of DNA sequences, in silico genomic approach)
- Microbiology (culture, maintenance and preservation of microorganisms)
**Who we are**
The groups _Applied Microbiology_ (head: Prof. Yvonne Mast) and _Actinobacteria_ (Head: Dr. Imen Nouioui), located at the Leibniz-Institut DSMZ in Braunschweig-Stöckheim, investigate the diversity and systematics of actinomycetes as antibiotic producers. We employ Modern Polyphasic Taxonomy, Genome Mining and Genetic Engineering techniques to determine the taxonomic rank of a strain and fully exploit the biosynthetic potential of actinomycetes.
**Beginn**: ab sofort
**Kontakt**: Leibniz-Institut DSMZ, Prof. Dr. Yvonne Mast (E-Mail: yvonne.mast(at)dsmz.de, Tel. 0531-2616-358), Dr. Imen Nouioui (E-Mail: imen.nouioui(at)dsmz.de, Tel. 0531-2616-369)
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