Research Assistant with Bachelor Degree

vor 1 Monat


Heidelberg, Baden-Württemberg, Deutschland Universität Heidelberg Vollzeit
Master Thesis Project: Engineering the Human Gamma-TuRC in Yeast

The gamma-TuRC is the principal microtubule organizer in human cells. We have resolved its structure using cryo-electron microscopy, revealing how over 40 gamma-TuRC subunits assemble into a 14-spoked helical structure.

Project Overview

In this master's thesis project, we will use budding yeast Saccharomyces cerevisiae as a model system to express all human gamma-TuRC subunits. First, we will assemble a chromosome derived from synthetic DNA fragments of the gamma-TuRC genes, in collaboration with the Zeiss Center of Synthetic Genomics at the ZMBH. Next, we will analyze the assembly of the gamma-TuRC using fluorescence microscopy, biochemical approaches, single-particle cryo-electron microscopy of purified gamma-TuRC, and cryo-electron tomography of yeast cells.

Research Objectives
  1. Assemble a chromosome derived from synthetic DNA fragments of the gamma-TuRC genes.
  2. Analyze the assembly of the gamma-TuRC using various microscopy and biochemical approaches.
  3. Engineer the gamma-TuRC and study the impact of these structural changes on the microtubule system.
Requirements

The position is available immediately, and the successful applicant will be supported by a Hiwi contract for six-eight months. Interested candidates should send their applications to Dr. Elmar Schiebel (schiebel.elmar@zmbh.uni-heidelberg.de) with a CV, a short letter of motivation, and the names of two referees.

Heidelberg University stands for equal opportunities and diversity. Qualified female candidates are especially invited to apply. Persons with severe disabilities will be given preference if they are equally qualified.



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