Research Assistant with Bachelor Degree
Vor 6 Tagen
Master Thesis Project: Engineering the Human Gamma-TuRC in Yeast
The Center of Molecular Biology and Center of Organismic Studies at Heidelberg University is seeking a highly motivated Research Assistant with a Bachelor Degree to join our team for a master thesis project.
The project focuses on engineering the human gamma-TuRC in yeast, a principal microtubule organizer in human cells. We have resolved its structure using cryo-electron microscopy, revealing how over 40 gamma-TuRC subunits assemble into a 14-spoked helical structure.
As a Research Assistant, you will use budding yeast Saccharomyces cerevisiae as a model system to express all human gamma-TuRC subunits. You will assemble a chromosome derived from synthetic DNA fragments of the gamma-TuRC genes, in collaboration with the Zeiss Center of Synthetic Genomics at the ZMBH.
Next, you will analyze the assembly of the gamma-TuRC using fluorescence microscopy, biochemical approaches, single-particle cryo-electron microscopy of purified gamma-TuRC, and cryo-electron tomography of yeast cells. In the second phase, you will tether the gamma-TuRC to the yeast spindle pole body for functional analysis.
This project is a collaboration between the laboratories of Drs. G. Pereira, S. Pfeffer, and E. Schiebel.
The position is available immediately, and the successful applicant will be supported by a Hiwi contract for six-eight months.
For more information, please visit our website.
Heidelberg University stands for equal opportunities and diversity. Qualified female candidates are especially invited to apply. Persons with severe disabilities will be given preference if they are equally qualified.
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