Phd Student Position in Bioengineering

Vor 3 Tagen

Heidelberg, Deutschland Universität Heidelberg Vollzeit

At the Center for Organismal Studies (COS) the following position is to be filled with 25,675 h/week:
**PhD Student Position in Bioengineering (f/m/d)**

Project: Correlative super-resolution light and X-ray microscopy to probe genome organisation

Chromatin organization plays an important role in the regulation and maintenance of genes in the normal biological process and in response to environment. The disruption of the organization of chromatin has a significant impact on many cell processes, often associated with many pathological processes such as aging and cancer. Recent advances in microscopy techniques open up new opportunities to directly visualize higher-order chromatin architecture, its compaction status and its functional states at nanometer resolution in the intact cells or tissue.

The project aims to develop correlative imaging based on super resolution fluorescence and soft x-ray microscopies in 3D via full-field rotation. Such full-field rotation will enable structural and functional studies to probe chromatin architecture in key cellular processes at nanometer resolution in 3D. Full-rotation, tomographic imaging is the unique way to obtain 3D cell structure with isotropic resolution. While means for full-rotation soft x-ray tomography are established, super-resolution techniques are typically limited to 2D information or confocal geometry. This project focuses on development of axial super-resolution microscopy which is compatible with thin glass-wall capillaries used in soft x-ray tomography. The project is part of the doctoral networks within Marie Skłodowska-Curie Actions with the reach portfolio in correlated microscopy across light, electrons and x-rays. The joint effort of 9 collaborating institutions (clexm.eu/) and possibility to exchange expertise and gain new experiences.

**Your tasks**:
Full-rotation, tomographic imaging is the unique way to obtain 3D cell structure with isotropic resolution. While means for full-rotation soft x-ray tomography are established, super-resolution techniques are typically limited to 2D information or confocal geometry. This project focuses on development of axial super-resolution microscopy which is compatible with thin glass-wall capillaries used in soft x-ray tomography.
- Development of axial super-resolution microscopy on thin glass-wall capillaries, including sample preparation, image acquisition and plunge freezing
- Development of fiducial markers and volume analysis pipelines to correlate soft x-ray tomography and super-resolution microscopy
- 3D mapping of chromatin compaction by means of correlative approach to understand formation of memory in immune cells

**Your profile**:

- M.S. in Physics, Engineering, Biophysics, Cell or Molecular Biology or related disciplines
- interest in imaging techniques, joy in solving technical puzzles
- experience in handling microscopes
- experience in data analysis or programming using MATLAB/Python/R
- good English language skills

Remuneration is based on TV-L (up to E 13, depending on qualifications). The position is limited for 3 years.

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