Senior Molecular Biologist

vor 4 Wochen

Stuttgart, Baden-Württemberg, Deutschland Robert-Bosch-Krankenhaus GmbH Vollzeit

Job Title: Senior Molecular Biologist

Job Summary:

We are seeking a highly motivated Senior Molecular Biologist to join our research team at the Robert-Bosch-Krankenhaus GmbH. The successful candidate will be responsible for utilizing modern sequencing-based molecular biology approaches to examine transcriptomic and gene regulatory mechanisms controlling tumor- and senescent cell identity and therapy response.

Key Responsibilities:

  • Utilize modern sequencing-based molecular biology approaches (RNA-seq, scRNA-seq, ATAC-seq, ChIP-seq) to examine transcriptomic and gene regulatory mechanisms controlling tumor- and senescent cell identity and therapy response.
  • Use bioinformatic analyses and interpretation of data to perform hypothesis testing and generation.
  • Perform multiplex immunofluorescence staining to confirm gene regulatory mechanisms in primary tumor tissue using multispectral imaging with an Akoya PhenoImager, perform Telomere-associated DDR foci (TAF) staining.
  • Perform tissue-based preparation of histological samples (FFPE tumor material) for single cell RNA-sequencing using the 10X Genomics technology.
  • Perform advanced cell culture experiments to examine the effects of drug treatment in various translational model systems.
  • Participation in internal or external research collaborations.
  • Documentation of work procedures, data acquisition and analyses.

Requirements:

  • Applicants must have completed a Master of Science in a related field (biology, molecular biology, biotechnology, bioinformatics or similar)
  • Experience with cell culture, transfections, stainings, etc.
  • Extensive molecular biology experience (real-time PCR, Western blot, etc.)
  • Experience with bioinformatic analyses (R, Python)
  • Strong work ethic and high level of self-motivation
  • Independent, goal-oriented and structured way of working and ability to work in a team
  • Confident written and spoken English skills

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