Technical Assistant

Technical Assistant (m/w/d) _
- in Single Cell Omics & TCR Validation _
- Full Time - Mainz_

We are looking for support in our **Clinical Cancer Genomics**unit to start as soon as possible. The successful

the development of novel immunotherapies against cancer and other diseases.

**Your tasks and responsibilities**:
Processing including dissociation of fresh, thawed or fixed healthy or patient tissues

Performance of routine cell culture work including maintenance and expansion of mammalian primary cells

(e. g. 2D and 3D tumor cell lines and TIL expansion)

Carrying out flow cytometry experiments and preparation of cell suspensions for sorting

Preparation of single cell or nuclei suspensions for NGS-based single cell sequencing (scRNA-seq, scTCR
- seq and multiomics) using the 10x Genomics platform

Generation of cDNA and ATAC libraries and preparation for sequencing on the Illumina platforms (MiSeq

or NovaSeq 6000)

Performance of functional TCR validation assays (e.g. ELISpot and killing assays) and immunogenic antigen

screens

Training of new employees, supervision of automated laboratory processes and writing standard operating

procedures (SOPs)

Timely generation of high-quality data and communication of results to the team

**What you bring:**

A completed professional training as BTA/MTA, biological laboratory assistant, or a Bachelor (B.Sc.)/Master

(M.Sc.) degree in natural sciences

Experience in molecular biology techniques (RNA extraction, PCR, NGS library preparation) is required

Experience in mammalian cell culture is required

Previous professional experience would be desirable

Experience in immunological techniques (e.g. ELISA, flow cytometry) would be of advantage

Any practice in processing primary samples (patient material, mouse samples) would be of advantage
Strong visual, verbal, and written communication skills in English and German as well as strong sense of

responsibility and high reliability are essential

Enthusiasm and curiosity for the diverse activities of our research institute as well as the ability to work in a

team completes your profile.
- PLATZHALTER ADMIN (M/F/D) _
- Vollzeit - Mainz _

**TRON** - Translational Oncology at the University Medical Center of the Johannes Gutenberg University Mainz non-profit GmbH

**We offer:**

A dynamic, innovative and creative research environment

An open, collegial and cordial working atmosphere in a respectful corporate culture

Highly interactive working environment across teams

A high degree of diversity in the workforce

Flat hierarchies

Performance-related remuneration and other benefits

The opportunity for personalised further training

Good transport connections by public transport and car as well as bicycle parking spaces

The opportunity for hybrid working

academic research with the requirements of quality-controlled industrial developments. At TRON, we share a

common mission to develop innovative solutions for the immunotherapeutic treatment of cancer, infectious

diseases and other serious diseases with high medicinal need for development.

TRON was founded in Mainz in 2010 and works in close cooperation with universities and hospitals as well as

with regional, national and international research institutions and pharmaceutical companies.

As part of our team, you have the opportunity to be at the forefront of translational science with us.

If all this appeals to you, we look forward to getting to know you.

Job-ID: _51012-25-01-TA_.

**TRON** - Translational Oncology at the University Medical Center of the Johannes Gutenberg University Mainz non-profit GmbH