Phd Student Prestart

Vor 5 Tagen

Mainz, Deutschland Institut für Molekulare Biologie gGmbH Vollzeit

**About us**:
**The position**:
Get research experience with the possibility to **prestart** a **PhD Student Position**

R-loops are three-stranded nucleic acid structures comprising an RNA hybrid and a displaced single-stranded DNA (ssDNA). The exposed ssDNA makes R-loops a hotspot for DNA modifications that can impact R-loop dynamics, gene regulation and genomic stability (Pubmed: 32005969). Recent studies have revealed that different nucleobases exhibit specific modifications in the context of R-loops, such as cytosine deamination to uracil cytosine oxidation to 5-formylcytosine, or guanosine oxidation to 8-oxoguanine. These modifications regulate R-loop stability and hence potentially affect genome stability and gene expression. R-loop-enriched modifications highlight the importance of understanding their regulatory roles and potential as therapeutic targets in diseases linked to genomic instability. The questions underlying this project are:

- How do specific DNA modifications within R-loops affect their structural stability and dynamics?
- What is the relationship between R-loop-associated DNA modifications and gene regulation?
- How do these modifications contribute to genomic instability and potentially to disease pathogenesis?

You will employ quantitative DNA-RNA immunoprecipitation sequencing to map the locations and frequencies of specific DNA modifications within R-loops across various genomic contexts. You may test the impact of these modifications on R-loop stability using CRISPR-Cas9 to engineer cell lines with and without specific modifications. You will then observe changes in gene expression, R-loop formation/disruption, and cellular phenotypes. You will conduct assays to measure rates of DNA damage, repair efficacy, and cell viability under conditions promoting R-loop formation and modification. This will help correlate specific modifications with increased genomic instability.

**Requirements**:

- Masters degree in a relevant area
- Good experience in molecular biology
- Good communication skills and good team spirit
- Ability to solve problems independently
- Fluent in English (spoken and written)

**Why join us?**:
We offer a stimulating, diverse and international research environment, with a pleasant working atmosphere, and informal culture. The institute is modern, well-equipped and centrally located with good public transport links and parking.

**Our offer**:

- Competitive salary and favourable pension scheme
- Advanced training opportunities
- Flexible working hours
- Employee events
- Job Ticket

**What else you need to know**:

- ** Starting Date**:By September 2025
- ** Deadline**:15th April 2025

**Have we sparked your interest?**:

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