Student (F/m/d) for a Master Thesis (Cefic-lri)

vor 3 Wochen


Duesseldorf, Deutschland IUF - Leibniz-Institut für umweltmedizinische Forschung GmbH Vollzeit

The working group “Alternative method development for environmental toxicity testing” led by Prof. Ellen Fritsche is looking for a student (f/m/d) for a Master Thesis with the title:
**Development of animal-free **_in vitro_** test methods to identify chemicals that disturb human brain development by interfering in radial glia
- and astrocyte-function.**

**The project**:
Environmental chemicals that interfere in human brain development cause developmental neurotoxicity (DNT) leading to developmental disorders such as autism or ADHD. These chemicals are currently identified in animal studies that are time
- and cost-intensive and raise severe ethical concerns. Therefore, alternative test methods have been developed that recapitulate key processes of human brain development _in vitro_. This project aims to close gaps within the available DNT_ in vitro_ test method portfolio by establishing novel test methods recapitulating the development of two crucial cell types within the human brain, radial glia (RG) and astrocytes, to improve the predictivity of _in vitro_ DNT testing. The new RG and astrocyte test methods will be based on a multicellular model using human neural progenitor cells (hNPCs) that has been intensively characterized in the Fritsche working group and applied in test methods studying chemical effects on NPC proliferation and differentiation into neurons and oligodendrocytes. Within this project culture conditions have been modified to enrich either proliferative migrating RG or maturing astrocytes, allowing the establishment of new RG- and astrocyte-specific test methods. As part of the test method set up, DNT-relevant test method endpoints have to be selected and both positive and negative controls have to be established. The new test methods modelling key neurodevelopmental functions of RG and astrocytes will then be challenged with a set of environmental chemicals consisting of chemicals evidently causing DNT in humans (positives) and chemicals that are harmless for the developing brain (negatives). You will work with an advanced multicellular cell model and learn different cell culture techniques when handling the 3D neurospheres. Moreover, the project includes different biochemical assays, immunocytochemical stainings, high content image analyses and gene expression analyses. Finally, your work will contribute to the development of novel animal-free test methods and the improvement of _in vitro_ DNT testing.

**Your profile**:

- Our working group is looking for a motivated student with a high level of commitment, fun at work, motivation, communication skills and team spirit.
- You have a bachelor degree in life science (biology, (bio)medical sciences, pharmaceutical sciences, bioengineering sciences, toxicology or equivalent).
- You have a good knowledge of the English language both written and oral.
- Experience with adherent and spheroid cell culture is desirable.
- Experience in the cultivation of primary cells and stem cells is advantageous.

**We offer**:
**Start**:
As early as possible.

Prof. Ellen Fritsche
IUF - Leibniz-Institut für umweltmedizinische Forschung
c/o Personalstelle
Auf’m Hennekamp 50
40225 Düsseldorf

Application documents submitted by post are not returned. Documents for applicants not considered are destroyed appropriately once the procedure is complete.



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