Senior Research Technician

vor 4 Wochen

Munich, Bayern, Deutschland Helmholtz Zentrum München Vollzeit

Flow Cytometry Specialist - Research Support


We are seeking a highly motivated and experienced Flow Cytometry Specialist to join our team at Helmholtz Zentrum München. As a key member of our Core Facility Flow Cytometry, you will be responsible for supporting all aspects of our core operation in cell sorting and analytical flow cytometry.

Key responsibilities include:

  • Daily operation of cell sorters and support users on analyzer platforms.
  • Train new users as appropriate in cell sorting and analysis.
  • Support creation of training content and information material.
  • Routine maintenance and quality controls of sorters and analyzers.
  • Troubleshoot technical problems of instruments and sample preparation, liaise with service engineers.
  • Establish new flow cytometry methodologies, instrumentation and workflows, also cross-platform.
  • Adhere to laboratory safety and standard operating procedures (self and users) and keep excellent written notes.
  • Support users in experimental planning, troubleshooting and data interpretation of very high end flow cytometry experiments.
  • Support the seamless operation of the core (e.g. ordering, billing, user management, user scheduling, supply management, keep lab in a state of good order and cleanliness).
  • Routine data backup from sorters and analyzers and maintenance of databases, routine scheduling of core facility services.

Requirements

  • Research/ lab technician (MTA/BTA/CTA) with at least 6 years of relevant flow cytometry experience, or Bachelor's/Master's degree in cell biology/immunology or a related field.
  • Significant experience working with flow cytometry technology, either in a core facility setting or in other roles.
  • Prior hands-on experience in operating and troubleshooting one or more of the following instruments: BD Aria III, BD FACSSymphony S6, BD Symphony A3 analyzer, Miltenyi MACSima analyzer.
  • Knowledge and understanding of complex high end panel design (> 20 parameters), optical configuration and compensation rules.
  • IT skills: MS Office (Excel, PowerPoint, Word) and at least one of the following softwares: Diva, FlowJo, FCSExpress.
  • Experience in general lab management (e.g. purchasing and stock controls, working with field service engineers, routine calibration and QC, waste management).
  • Experience in writing, managing and reviewing SOPs.
  • Skills in molecular biology, cell culture or immunology assays (e.g. intracellular cytokines, activation, secretion assay, Treg supression assay).
  • Experience in training users in flow cytometry applications and use of instrumentation.
  • Ability to work independently with minimal supervision and as part of a team.
  • Strong communication and organizational skills and an open, proactive mindset and clear customer service orientation.
  • Excellent written and oral communication skills in English, German highly desirable.

Desirable qualifications

  • Experience working at Containment Level 2 (BSL2).
  • Experience in high-end panel design for high throughput imaging or spectral analysers.
  • Experience with single cell and spatial biology applications.
  • Skills in microscopy, histology and high dimensional flow cytometry data analysis.

Benefits

Helmholtz Zentrum München is actively committed to diversity and inclusion in practice and is sustainably committed to equality. We are committed to promoting a culture of diversity and welcome applications from talented people regardless of gender, cultural background, nationality, ethnicity, sexual identity, physical abilities, religion or age. Qualified applicants with physical disabilities will be given preference.

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