Research Assistance

RESEARCH ASSISTANCE (M/F/d) _
- Full time - Mainz_

We are looking as soon as possible for support in our **MetaboEdit team in the Vaccine and Cellular**

develop innovative cancer immunotherapies based on understanding and exploiting of cancer and immune

cells metabolism

Our research interests includes identifying metabolism targets via CRISPR screening as well as manipulation of

tumor microenvironment to improve translational immunotherapy approaches such as RNA-based

vaccinations. A variety of different projects is carried out in a multidisciplinary team within TRON and with

external collaborators. Methodologically we primarily focus on gene-manipulation of cancer and immune cells

with extensive in vitro analysis such as examinations of the metabolism and cytotoxicity, complemented with

in vivo investigation of the tumor microenvironment and immunogenicity of combination treatments.

**Your tasks and responsibilities**:

Planning, conducting and analysis of preclinical experiments in the field of cancer immunotherapy and

metabolism (u.a. Seahorse, bioluminescent assays)

Generation and validation of gene-edited cell lines via CRISPR/Cas9 systems

Planning, conducting and analyzing immunological in vitro assays based on multicolor flow cytometry and

co-culture cytotoxicity assays.

Establishment and standardization of new methods

Writing standard operating procedures (DE & EN) and preparation of experimental reports

Pro-active contribution to tasks of general laboratory organization

**What you bring: **

A master degree in life sciences or several years of experience as technical assistant in the field of cell

biology (or equivalent) are favorable

Any practical experience in conducting in vitro experiments using cell culture and primary cells isolated

from organs

Experience in performing in vitro assays based on multicolor flow cytometry techniques and immunological

methods such as ELISA and co-culture assays are a plus

Basic knowledge in conducting metabolic assays would be a plus

Strong verbal and written communication skills in both German and English language

Well-structured and organized working methods and an excellent time management

Enthusiasm and curiosity for the diverse activities of our research institute as well as the ability to work in a

team completes your profile.
- PLATZHALTER ADMIN (M/F/D) _
- Vollzeit - Mainz _

**TRON** - Translational Oncology at the University Medical Center of the Johannes Gutenberg University Mainz gGmbH (non-profit)

**We offer: **

A dynamic, innovative and creative research environment

An open, collegial and cordial working atmosphere in a respectful corporate culture

A high degree of diversity in the workforce

Flat hierarchies

A performance-related remuneration and other benefits

The opportunity for personalised further training

An excellent transport connection by public transport and car, parking spaces, secure bicycle parking

The opportunity for hybrid working on a daily basis

TRON is an internationally recognised institute for translational research. We combine the strengths of

academic research with the requirements of quality-controlled industrial developments. At TRON, we share a

common mission to develop innovative solutions for the immunotherapeutic treatment of cancer, infectious

diseases and other serious diseases with high medicinal need for development.

TRON was founded in Mainz in 2010 and works in close cooperation with universities and hospitals as well as

with regional, national and international research institutions and pharmaceutical companies.

As part of our team, you have the opportunity to be at the forefront of translational science with us.

If all this appeals to you, we look forward to getting to know you.

Job-ID: _52009-23-03-TA_.

**TRON** - Translational Oncology at the University Medical Center of the Johannes Gutenberg University Mainz non-profit GmbH