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Postdoc (F/m/x) Position
vor 4 Monaten
as of November 1, 2023
full-time; initially limited until October 31, 2027 (third party funded project)
Your salary will be based on TV-L
- Your tasks
Isolation of murine tissue samples for sequencing and metabolomic approaches
Isolation and culture of mouse primary cells as ex-vivo system
Perform diverse sequencing methods (EU-, ATAC-, ChIP-, Cut&Break-seq)
Independent data analysis
Preparation of results for publications and presentations
- Your Profile
A Ph.D. degree with advanced training in molecular and cellular biology, animal genetics and/or pathology
Advanced experience in mouse genetics and tissue handling, and cell culture is essential
FELASA-B certification (or equivalent) is required
Strong expertise in sequencing techniques and data handling
A research background in physiology, metabolism or aging is a plus
Ability to independently drive research projects and animal experiment proposals (according to german law)
High level of motivation, enthusiasm, resilience and positivity
Ability and willingness to work in a collaborative team
Excellent communication in English (written and speech)
- Our Offer
What we have to offer is a vibrant work environment with strong affiliation within the FOR5504 research unit. This offers the opportunity of mentoring and interactions with several global scientific leaders in their respective fields. In addition, the highly collaborative research environment in Cologne provides us with access to state-of-the-art core facilities, including the entire OMICS-spectrum and imaging techniques.
- Your future with us
Working at the University Hospital Cologne and the Medical Faculty means helping to shape the future - the future of medicine, of patients and, of course, your own future. You benefit from 60 clinics and institutes as well as numerous other departments and facilities and more than 12,000 jobs. The Faculty of Medicine of the University of Cologne and the University Hospital Cologne assume important social tasks in research, teaching and patient care. A close network with many university and non-university partners guarantees an internationally successful science and the excellent education of our students.
- Your future in detail
The subproject aims at gaining a completer insight of the impact of genome instability (GI)-related transcriptional stress (TS) on systemic aging and underlying mechanisms. The group further aims to characterize the genomic, transcriptional and metabolic burden of increasing GI and TS in different postmitotic tissues and to examine the effects of dietary-restriction-induced metabolic redesign.
To achieve this, state-of-the-art mammalian GI models will be employed in combination with multi-OMICS- and high-throughput sequencing approaches, and in-vitro and ex-vivo techniques.
**Selected publications**:
Gyenis A, [ ], Hoeijmakers JHJ, Pothof J. Genome-wide RNA polymerase stalling shapes the transcriptome during aging. Nat Genet. 2023 Feb;55(2): 268-279. doi: 10.1038/s41588-022-01279-6. Epub 2023 Jan 19. PMID: 36658433; PMCID: PMC9925383.
Schumacher B, Pothof J, Vijg J, Hoeijmakers JHJ (2021). The central role of DNA damage in the ageing process. Nature. 2021 Apr;592(7856): 695-703. doi: 10.1038/s41586-021-03307-7. Epub 2021 Apr 28. PMID: 33911272.
Lans H, Hoeijmakers JHJ, Vermeulen W, Marteijn JA (2019). The DNA damage response to transcription stress. Nat Rev Mol Cell Biol. 2019 Sep 26. doi: 10.1038/s41580-019-0169-4. [Epub ahead of print] PMID: 31558824
Milanese C, [ ], Hoeijmakers JHJ, Mastroberardino PG (2019). DNA damage and transcription stress cause ATP-mediated redesign of metabolism and potentiation of anti-oxidant buffering. Nat Commun. 2019 Oct 25;10(1): 4887. doi: 10.1038/s41467-019-12640-5. PMID: 31653834
Vermeij WP, [ ], Vijg J, van Steeg H, Hoeijmakers JHJ. Restricted diet delays accelerated ageing and genomic stress in DNA-repair-deficient mice. Nature. 2016 Sep 15;537(7620): 427-431. doi: 10.1038/nature19329. Epub 2016 Aug 24. PMID: 27556946; PMCID: PMC5161687.
The position is suitable for staffing with part-time employees.
**Contact**:
Simon Uszkoreit
+49 221 478-84203
**Application deadline**: 01.10.2023
**Postal address**:
Universitätsklinikum Köln AöR
PA29
Geschäftsbereich Personal
