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Doctoral Researcher
vor 4 Monaten
**Job advertisement no. 016/2024
The department **MICA - Anti-infectives from Microbiota**, led by Prof. Christine Beemelmanns at the Helmholtz
- Institute for Pharmaceutical Research Saarland (HIPS) in Saarbrücken, is offering a position as
**Doctoral Researcher (f/m/d)**
**Project title: Design and metabolic analysis of bacterial communities (META-NAT)**
The Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) focusses on identifying and developing
new treatment options for infectious diseases with an emphasis on natural product research. HIPS was jointly
started in August 2009 by the HZI in Braunschweig and Saarland University on Campus Saarbrücken.
In 2015 HIPS moved into a new 4500 m2 research building in which currently 220 international employees work.
HIPS represents the first and only publicly funded extra-university research unit in Germany dedicated to
pharmaceutical research. The Institute collaborates with universities and various industries both nationally and
internationally. Researchers in the department **Anti-infectives from Microbiota** are analyzing and identifying
and methods mainly from the field of biotechnology, microbiology, molecular biology and biochemistry.
The **PhD project META-NAT** focusses on the identification of novel bacterial natural product producers and the
metabolic analysis of communities thereof. The project will make use of state-of-the art microbiological
techniques and sequencing technologies, and combine bacterial cultivation with natural product discovery efforts
to understand the natural functions of secondary metabolite.
The PhD project is structured in **three interconnected research pillars**:
1. **Isolation and genome sequencing of (marine) bacterial symbionts**
- Isolation and cultivation of bacterial strains
- Testing of different microbial (co-)cultivation techniques
- Isolation of DNA and whole genome sequencing
**2. Metabolic analysis of (marine) wild-type natural product producers**
- Focus on the secondary metabolome of bacterial producers
- In collaboration, isolation of novel natural products
- Utilizing high-resolution mass spectrometry to monitor and optimize product formation
**3. Design and analysis of bacterial co-cultures**
- Design and metabolic analysis of multitrophic bacterial co-cultures.
- Optimizing cultivation conditions of bacterial co-cultures
- If possible, establish RNA-sequencing protocols of bacterial co-cultures
genome-sequencing efforts for genome mining and comparative studies. Analysis of fermentation conditions and
co-cultures will ignite studies into the natural product repertoire. A particular focus should be set on multi-trophic
instruments and use this knowledge for natural product identification efforts. This multi-faceted approach will
biological and analytical tools for the identification of novel natural products. This multi-faceted approach will
biological and analytical tools for the identification of novel natural products.
**Qualifications**:
Master degree or equivalent in Life Sciences, Biotechnology, Pharmacy Chemistry, or related fields.
Strong hands on experience in laboratory and in particular microbiological cultivation techniques.
General understanding of molecular biological approaches, sequencing technologies, chemical and
biochemical transformations
Ability to pay attention to details, pursue research independently and work in a goal-oriented manner.
Willingness to work in a plural, collegial, international and interdisciplinary environment.
Excellent English communication skills (written and spoken); very good skills in scientific writing
**Advantageous for this position**:
Experience in next-generation sequence methods/techniques
Experience in DNA isolation, PCR and cloning techniques
Isolation of bacterial strains, microbial cultivation techniques, phylogenomic analyses
Disabled persons are given preference in the case of equal professional qualification. The HIPS aims for
professional equality between women and men. The position is suitable for part-time work.
**We offer**:
Modern laboratories and state-of-the-art instrumentation, a dynamic and international research
environment
Extensive further training opportunities and the opportunity to enroll in a structured PhD program
Unique network of excellent partners to support your research endeavors
30 days vacation (24.12. & 31.12. are considered as completely free days)
An annual additional payment (Weihnachtsgeld) analogue to - 20 TVöD
Social security included
Flexible working hours
A corporate culture of appreciation and promotion of equal opportunities
Support for a better balance between work and private life through our family office
**Starting date: as soon as possible, **2024 - initial contract for 3 years.
**Salary**:alike** **E13 TVöD/Bund (55%)
**Probation period**:6 months_ _
**Working place**:Saarbrücken
**Published: 22 February, 2024**
**Closing date: 24 March, 2024**
For furt
