Phd Position in Single Cell Immunobiology

vor 2 Wochen

Freiburg, Deutschland Universitätsklinikum Freiburg Vollzeit

The Department of Internal Medicine II at the University Hospital in Freiburg is investigating the role of gamma delta T cells in intestinal inflammation and is looking for one for this purpose

**PhD Position in Single Cell Immunobiology (M/F/d)**:

- We are a small, dedicated group of scientists working to understand gamma delta T cell biology using cutting-edge single-cell technologies. We are seeking a highly motivated and skilled individual to join our team as a PhD student as part of the collaborative research center (CRC1160) to investigate the role of gamma delta T cells in intestinal inflammation in mice and humans.
**Project description**
- PhD position in Single-Cell Analysis of GAMMA DELTA T cell biology in INTESTINAL inflammation- The project involves substantial collaboration with other scientists of CRC 1160, who will perform experiments on DSS-induced colitis to investigate the immune landscape during inflammation in the intestine. The main aim of the project is to analyze single-cell multimodal datasets (including gene expression, chromatin accessibility, T-cell receptor sequencing, and cell-surface protein profiling) to understand the role of immune cells and their interactions with epithelial cells in intestinal homeostasis and inflammation, with a special focus on gamma delta T cells.**What we offer**:

- a four-year contract based on the German public service pay scale
- a flexible environment where you will have the possibility to develop your own skillsets
- mentorship on how to be productive and successful, resulting in several publications without micromanaging
- joining date is flexible

**Responsibilities**:

- perform single-cell RNA sequencing and other single-cell multimodal profiling using microfluidics and split and pool technologies
- intensively perform computational data analysis of the generated datasets in collaboration with other scientists
- present the biological interpretation of datasets in a comprehensive way during lab meetings, retreats, and meetings with collaboration partners

**Requirements**:

- Master's degree in biology, molecular medicine, or immunology
- experience in basic molecular and cellular biology techniques such as cell culture, FACS, qPCR, etc.
- basic knowledge of R and/or Python
- strong interest and motivation in analyzing your own datasets as the position is mainly computational
- excellent communication skills in English
- ability to work independently and to derive creative hypotheses from the data for further validation experiments

**Required documents**:

- motivation letter and CV
- Bachelor's and master's degree certificates
- transcript records
- minimum two letters of recommendation

**Selection procedure**:

- presentation of Master's thesis research
- presentation of a paper related to single-cell biology
- the applicant will be given a sample dataset to analyze and will be asked to present the data

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