Phd Position in Structural Biology

vor 4 Wochen


Heidelberg, Deutschland Deutsches Krebsforschungszentrum Vollzeit

**Position**:PhD Position in Structural Biology & Enzymology of Protein Kinase Regulation by RNA Helicases**

**Department**: Molecular Embryology

**Code number**:2023-0217

The German Cancer Research Center is the largest biomedical research institution in Germany. With more than 3,000 employees, we operate an extensive scientific program in the field of cancer research.

For the Division of Molecular Embryology we are seeking a PhD student in the field of structural biology and enzymology to start in November 2023.

**Job description**:
Wnt signaling is a key process in cell differentiation and oncogenesis. Casein kinase 1 (CK1) regulates Wnt signaling pathway at multiple levels. The lab of Prof. Dr. Christof Niehrs (Division of Molecular Embryology) has identified the DEAD-box RNA helicase DDX3 as effector of the Wnt-β-catenin network, where it acts as an allosteric activator of CK1 (Cruciat et al (2013); Fatti et al (2023). Both DDX3 and CK1 are oncogenes and hence their investigation is of relevance for the development of novel cancer therapeutics. Our data indicate that DDX3 allosterically activates CK1. You will follow up on the kinetic mechanism of this new regulatory paradigm between kinases and helicases by conducting recombinant protein expression, enzyme kinetics, biophysical measurements including stopped flow spectroscopy, and protein crystallography to provide a mechanistic understanding.

References:
Cruciat et al. (2013). RNA helicase DDX3 is a regulatory subunit of casein kinase 1 in Wnt-β-catenin signaling. Science 339, 1436-1441.

Fatti et al. (2023) DEAD-box RNA Helicases Act as Nucleotide Exchange Factors for Casein Kinase 2. Sci Signal. doi: 10.1126/scisignal.abp8923.

**Requirements**:

- & Masterzeugnis), a cover letter stating research and career interests, expected availability date, a list of publications and details of two references.

**We offer**:

- Excellent framework conditions: state-of-the-art equipment and opportunities for international networking at the highest level
- Access to international research networks
- Doctoral salary with the usual social benefits
- 30 days of vacation per year
- Flexible working hours
- Possibility of mobile work and part-time work
- Family-friendly working environment, e.g. parent-child room, advisory services
- Sustainable travel to work: subsidized Germany job ticket
- Unleash your full potential: targeted training and mentoring through the DKFZ International PhD Program and DKFZ Career Service
- Our Corporate Health Management Program offers a holistic range of services for your well-being

**Important notice**:
**Earliest Possible Start Date**: 01.11.2023

**Duration**: The position is limited to 3 years with the possibility of prolongation.

**Application Deadline**:18.07.2023

**Contact**:
Prof. Dr. Christof Niehrs
Phone +49 (0)6221-42 4690

The DKFZ is committed to increase the proportion of women in all areas and positions in which women are underrepresented. Qualified female applicants are therefore particularly encouraged to apply.



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